Effect of Galardin as Matrix Metaloproteinase Inhibitor on the Dentin Bonding Durability of Old and Current Generations of Adhesive Systems

Objectives: This study aimed to evaluate the effect of Galardin as a specific matrix metalloproteinase (MMP) inhibitor on dentin bonding durability of different generations of adhesive systems. Materials and Methods: An in vitro study was performed on 32 extracted sound human molars. The occlusal enamel was removed prior to random assignment of the teeth into 8 groups by adhesive type and application/no application of Galardin. Each group was subdivided into 2 subgroups with/without aging. Adhesives were applied after the application of Galardin in the respective groups according to the manufacturer's instructions, and composite resin was incrementally built up to a height of 6mm. After performing the aging protocols for the respective subgroups, the teeth were sectioned into bar-shaped samples with a cross-sectional area of 1mm². The micro-tensile band strength (µTBS) test was then performed. Data were analyzed by three-way ANOVA, Bonferroni and Games-Howell post-hoc tests, and Chi-square test (alpha=0.05). Results: The type of adhesive system, aging, and Galardin application had statistically significant effects on µTBS (P<0.001). Application of Galardin increased the µTBS (P<0.001) while aging decreased it (P<0.001). The interaction effect of aging and type of adhesive system on µTBS was also statistically significant (P<0.001). The other two-way and three-way interaction effects were not statistically significant (P>0.05). Conclusion: Application of Galardin increased the µTBS values both immediately and after 6 months irrespective of the adhesive system type. Aging significantly decreased the µTBS. Type of adhesive also significantly affected the µTBS.