Uncovering new antibiofilm roseabols from marine Clonostachys rosea through structural and multiomics approaches
Through a combined approach, that involved the one-strain-many-compounds (OSMAC) culturing approach, metabolomic and genomic studies, and bioassay-guided purification we disclose the marine fungus Clonostachys rosea as producer of a family of peptaibols with new structural features. HRMS/MS, NMR, and advanced Marfey’s analysis enabled the structural elucidation of two major compounds of the roseabol family, named roseabols B ( 1 ) and C ( 2 ). Molecular networking further revealed a highly diverse and microheterogeneous peptaibol profile. Biological evaluation highlighted the ability of the isolated peptaibols to inhibit biofilm formation against Escherichia coli and Candida albicans . Overall, these studies demonstrate the effectiveness of metabolomics-driven strategies in discovering novel natural products and identify marine-derived peptaibols as promising antibiofilm agents.
Authors
- Valentina Catania (ORCID: https://orcid.org/0000-0002-9439-0980)
- Massimiliano Fenice (ORCID: https://orcid.org/0000-0001-8504-0885)
- Maria Valeria D’Auria (ORCID: https://orcid.org/0000-0003-3450-7304)
- Domenico Schillaci (ORCID: https://orcid.org/0000-0003-2416-0474)
- Donatella de Pascale (ORCID: https://orcid.org/0000-0002-6732-0901)
- Donatella Ferraro (ORCID: https://orcid.org/0000-0003-0911-9289)
- Simona De Marino (ORCID: https://orcid.org/0000-0002-0300-5048)
- Martina Braconcini (ORCID: https://orcid.org/0009-0000-6742-6565)
- Carmen Festa (ORCID: https://orcid.org/0000-0001-8879-4332)
- Marcella Pasqualetti (ORCID: https://orcid.org/0000-0002-2032-5596)
- Gerardo Della Sala (ORCID: https://orcid.org/0000-0001-8957-3259)
- Mattia Cammarota
Institutions
- Università degli Studi della Tuscia (IT)
- Stazione Zoologica Anton Dohrn (IT)
- University of Naples Federico II (IT)
- University of Palermo (IT)
Publication Details
- Journal
- Natural Products and Bioprospecting
- Published
- 2026-11-11
- DOI
- https://doi.org/10.1007/s13659-026-00659-3
- Primary Topic
- Microbial Natural Products and Biosynthesis
- Type
- article
- Field-Weighted Citation Impact
- 0.00