Annotation of Specific Genes in Olimarabidopsis pumila

Abstract Olimarabidopsis pumila ( O. pumila ) is an annual spring ephemeral mustard plant that possesses adaptations to its semi-arid and salinized desert environments, affording it high salinity tolerance and the ability to maintain efficient photosynthesis under high-intensity solar radiation [1]. Four genes were identified between base pairs 7,500,000 and 8,000,000 on contig tig00002140 using the Genomic Sequence Annotation Server (GenSAS) and AUGUSTUS [2, 3]. Sequence conservation was assessed using the National Center for Biotechnology Information (NCBI) Protein Basic Local Alignment Search Tool (BLASTP) and Multiple Sequence Alignment (MSA) Viewer [4, 5]. Localization was determined using WoLF PSORT, SignalP 6.0, and Transmembrane Hidden Markov Models (TMHMM) 2.0 [6, 7, 8]. Lastly, structural analysis was configured using InterProScan, AlphaFold, STRING, and the combination of UniProt and ColabFold [9, 10, 11, 12, 13]. The four gene models were predicted to encode a DnaJ‑family chaperone that assists in protein folding, an indole‑3‑glycerol phosphate synthase used in tryptophan biosynthesis, a ribosomal RNA‑processing protein involved in rRNA maturation, and a protein with a GEF domain that is annotated as a ubiquitin ligase [9]. These four gene models are candidates for roles in protein translation, cellular homeostasis, signaling, and hormone biosynthesis that may contribute to plant growth and stress resilience.