Green and simple rrs spectroscopic strategy based on xanthene-based dye; Acid Red 51 for eletriptan quantification in pharmaceuticals and biological samples

Abstract Eletriptan tablets are a targeted pharmaceutical agent that acts as an agonist for the 5-hydroxytryptamine1 receptor subclassification, mostly employed to alleviate acute migraine episodes. As a member of the triptan class, Eletriptan functions by constricting cerebral blood vessels and decreasing the release of chemicals that cause light and sound sensitivity, nausea, and headache discomfort. Its efficacy in alleviating migraine symptoms makes it a valuable option for individuals seeking rapid and effective relief. A sustainable, rapid, one-pot resonance Resonance Rayleigh Scattering spectrometric technique was created for estimating out Eletriptan in pharmaceutical tablets and biological matrices. Upon mixing Eletriptan with the fluorescent ligand Acid Red 51 in an acidic buffer, a marked increase in resonance Resonance Rayleigh Scattering intensity was immediately observed. This phenomenon was ascribed to the selective formation of an ion-pair complex between the cationic Eletriptan drug and the anionic dye, which amplified the Resonance Rayleigh Scattering signal through enhanced scattering interactions. The RRS intensity showed a straight-line connection with Eletriptan concentration between 50 and 1500 ng/mL, allowing for accurate measurement. Key experimental parameters, including pH, ligand concentration, and buffer volume, were systematically optimized to maximize the sensitivity of the Eletriptan –Acid Red 51 system. The method adhered to International Council for Harmonisation validation criteria, achieving detection and quantification limits of 15.7 ng/mL and 47.8 ng/mL, respectively. Effective implementation to pharmaceutical and biological samples confirmed the method’s accuracy and reproducibility, with recovery rates aligning with acceptable analytical standards. Notably, the approach minimizes solvent consumption and hazardous waste generation, aligning with green analytical principles. By integrating simplicity, environmental sustainability, and high sensitivity, this Resonance Rayleigh Scattering-based technique emerges as a robust alternative for routine analysis of Eletriptan in quality control and biomedical research.