Mouse Brain Processing for Laser Capture Microdissection (LCM) of Catecholaminergic Brain Regions v1
This protocol describes the preparation of mouse brain tissue for laser capture microdissection (LCM) of catecholaminergic brain regions including the substantia nigra (SN), ventral tegmental area (VTA), and locus coeruleus (LC).The procedure preserves RNA integrity for downstream transcriptomic analyses including microarrays and qPCR. The protocol was optimized for the study of neuromelanin-containing transgenic mice and wild-type controls.
Authors
- Ariadna Laguna (ORCID: https://orcid.org/0000-0002-9732-6677)
- Miquel Vila (ORCID: https://orcid.org/0000-0002-1352-989X)
- Núria Peñuelas (ORCID: https://orcid.org/0000-0001-6443-9790)
Publication Details
- Published
- 2026-11-11
- DOI
- https://doi.org/10.17504/protocols.io.xmvm7o1pnv3p/v1
- Primary Topic
- Molecular Biology Techniques and Applications
- Type
- preprint