A Centrifuge-Free Method for Cell Culture Passaging and Collection Compatible with Automated Liquid Handling Systems: A Conceptual Methodology Framework

Standard cell culture passaging and collection workflows predominantly rely on centrifugation to pellet cells and replace liquid media. While effective, centrifugation introduces severe mechanical complexity, high hardware costs, and severe integration bottlenecks within automated liquid handling workstations. This paper introduces a conceptual, centrifuge-free methodology that replaces conventional centrifugal sedimentation with controlled size-exclusion filtration using standard syringes and syringe-mounted porous membranes. By standardizing a multi-step fluidic workflow—from cell dissociation, aspiration, and gentle membrane filtration to filter removal and replating—this approach provides an elegant solution for closed-system, low-shear cell handling. Furthermore, we outline its architectural compatibility with high-throughput laboratory automation (such as the Hamilton Microlab STAR platform), eliminating the need for robotic centrifuge integration, automated doors, and complex plate-balancing mechanisms. This conceptual framework establishes a streamlined foundation for future automated cell culture engineering.