Biosynthesis, Characterization and Antibacterial Properties of Silver Nanoparticles From the Edible Mushroom Pleurotus Ostreatus

Abstract The most common sterilising silver nanoparticles used in consumer and medical products include fabrics, food storage bags, refrigerator surfaces and personal care products. This study exhibits the synthesis of silver nanoparticles using Pleurotus ostreatus. The biosynthesized Ag-NPs were characterized using a UV-Vis spectrophotometer, Fourier transform infrared spectroscopy (FTIR), Scanning electron microscope (SEM), Transmission electron microscope (TEM), X-ray diffractometer (XRD) and antibacterial activity. The maximum absorption peak was measured in the UV-Vis spectra of silver nanoparticles at 460 nm. The next analysis is performed with a comparison of control and concentration 10mM. The FTIR were detected by the presence of functional groups necessary for the conversion of silver nitrate to silver ions. The formation of silver nanoparticles was observed by SEM analysis of the brown colour stable samples, while silver nitrate-treated samples revealed well-dispersed nanoparticles. The TEM images revealed that the size of Ag-NPs was found to be about 50 and 100 nm. Ag-NPs, XRD pattern showed 2θ values, which are related to the silver (111) crystalline phase index. Aeromonas hydrophila, Bacillus cereus, B. subtilis, Escherichia coli, Klebsiella pneumoniae, Vibrio cholera and Staphylococcus aureus were the microbes selected for the current study for the antibacterial activity of the silver nanoparticles was performed by well diffusion method. The maximum zone of inhibition was found in the control (10.16±0.18mm) at Klebsiella pneumoniae and concentration 10mM (12.20±0.08mm) at Bacillus cereus and least against the control (7.30±0.22mm) at E.coli and concentration 10mM (7.67±0.72mm) at Klebsiella pneumoniae. This study concluded that the silver nanoparticles have a significant amount of potential as an antimicrobial compound against the pathogenic microorganisms studied and can be used to treat infectious diseases caused by bacteria. Silver nanoparticles have a major role on nanotechnology and nanomedicine.