MiR-149-5p inhibits the proliferation and migration of Barrett’s esophagus cells with potential involvement of IL6

Abstract Background Barrett’s esophagus (BE) arises from chronic inflammation caused by acid reflux. This precancerous condition increases the risk of esophageal adenocarcinoma. Aims This research focused on the functional involvement of miR-149-5p in BE‑related cellular pathological events. Methods MiR-149-5p and IL6 were detected in tissues from non-BE and BE patients. CP-A and CP-B cells were exposed to bile acid (BA) to simulate gastroesophageal reflux in BE cells. The levels of miR-149-5p and IL6 were validated by RT-qPCR. COX2 and IL8 levels in the cells were measured using ELISA analysis. Cell proliferation and migration were assessed by CCK-8 and Transwell assays, respectively. The targeting relationship between miR-149-5p and IL6 was verified through correlation analysis, dual-luciferase reporter assay, and rescue experiments. Results Compared with non-BE patients, miR-149-5p expression was significantly downregulated in BE patient tissues. Following BA stimulation, miR-149-5p expression decreased while IL6 expression increased in CP-A and CP-B cells. MiR-149-5p overexpression abrogated BA-induced enhancement of CP-A and CP-B cell proliferation, migration and IL8 secretion. Rescue assays showed that IL6 overexpression partially reversed miR‑149‑5p‑mediated suppression of cellular proliferative phenotypes and STAT3 phosphorylation. Conclusion MiR-149-5p may regulate IL8 expression and STAT3 phosphorylation via potential involvement of IL6, and influencing esophageal epithelial cell malignant phenotypes.