Pengaruh Pemberian Albumin Ikan Gabus terhadap Jumlah Sel Fagosit pada Mencit BALB/c yang Dibuat Luka Eksisi

Snakehead fish albumin (Channa striata) is a natural protein source that is believed to play a role in the wound-healing process. Albumin contains lysine, leucine, and valine and functions to modulate the local immune response. Phagocytic cells, particularly macrophages and neutrophils, play an important role in the inflammatory phase of wound healing. This study aimed to analyze the effect of snakehead fish albumin administration on the number of phagocytic cells in BALB/c mice with excision wounds. This laboratory experimental study used a post-test only control group design. A total of 24 male BALB/c mice were divided into four groups: K1 negative control, K2 positive control treated with povidone-iodine (Betadine), K3 treated with 3.1% snakehead fish albumin, and K4 treated with 6.2% snakehead fish albumin. Excision wounds were created on the dorsal region of the mice. Two mice from each group were observed and euthanized on days 3, 7, and 14. The number of phagocytic cells was then counted using Hematoxylin and Eosin (H&E) stained preparations in five microscopic fields. Data were analyzed using the Kruskal–Wallis test followed by pairwise comparison. There was no significant effect on the number of phagocytic cells among the treatment groups (H = 1.160; p = 0.763). On the contrary based on the observation periods of days 3, 7, and 14, a significant difference phagocytic cell was found, particularly between day 3 and day 7 (p = 0.010). Administration of snakehead fish albumin did not have a significant effect on the number of phagocytic cells; however, an increase in the number of phagocytic cells was observed between days 3 and 7, There is an increase in the number of phagocytic cell due to the proliferative phase of wound healing.