[Re] Independent Python replication of E. coli starvation-stress reporter analyses Partial computational replication and sensitivity of source-defined analyses

We independently replicate the exported numerical analyses underlying Figure 6B/C/D of Gervais et al.’s Escherichia coli starvation and oxidative-challenge study and examine how the observational hierarchy affects their interpretation. The recovery panel contains 1,153 software-defined cycles, including 245 unresolved outcomes; 1,145 recovery IDs appear in the pre-challenge reporter table, with 1,139 carrying usable measurements of both reporters. Treatment arms form two disjoint sets of experiment dates, making a four-arm comparison distinct from a within-date contrast. Under unrestricted binary completion, the pooled CAM-after-minus-CAM-throughout contrast remains positive by at least 0.210 percentage points, but both September 2022 dose strata admit an ordering reversal; the June 2023 dose strata remain positive. Equal weighting of 37 source-identifier partitions is a separate descriptive estimand: its small cross-date order reversal requires 130 simultaneous, oppositely assigned unresolved labels. We reproduce the author’s exported reporter summaries, recover panel-specific sample definitions, and provide runnable source checks, exact finite-record sensitivity intervals and figure reconstruction. The work clarifies when a pooled software-level ranking is robust, and what assumptions are required to compare it across observation sets and source dates.

Authors

Publication Details

Journal
Zenodo (CERN European Organization for Nuclear Research)
Published
2026-11-10
DOI
https://doi.org/10.5281/zenodo.24115005
Primary Topic
Bacterial Genetics and Biotechnology
Type
preprint
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preprint

[Re] Independent Python replication of E. coli starvation-stress reporter analyses Partial computational replication and sensitivity of source-defined analyses

Rongzhen Dai
Zenodo (CERN European Organization for Nuclear Research)
Bacterial Genetics and Biotechnology
preprint

[Re] Independent Python replication of E. coli starvation-stress reporter analyses Partial computational replication and sensitivity of source-defined analyses

Rongzhen Dai
preprint en

Abstract

We independently replicate the exported numerical analyses underlying Figure 6B/C/D of Gervais et al.’s Escherichia coli starvation and oxidative-challenge study and examine how the observational hierarchy affects their interpretation. The recovery panel contains 1,153 software-defined cycles, including 245 unresolved outcomes; 1,145 recovery IDs appear in the pre-challenge reporter table, with 1,139 carrying usable measurements of both reporters. Treatment arms form two disjoint sets of experiment dates, making a four-arm comparison distinct from a within-date contrast. Under unrestricted binary completion, the pooled CAM-after-minus-CAM-throughout contrast remains positive by at least 0.210 percentage points, but both September 2022 dose strata admit an ordering reversal; the June 2023 dose strata remain positive. Equal weighting of 37 source-identifier partitions is a separate descriptive estimand: its small cross-date order reversal requires 130 simultaneous, oppositely assigned unresolved labels. We reproduce the author’s exported reporter summaries, recover panel-specific sample definitions, and provide runnable source checks, exact finite-record sensitivity intervals and figure reconstruction. The work clarifies when a pooled software-level ranking is robust, and what assumptions are required to compare it across observation sets and source dates.

Zenodo (CERN European Organization for Nuclear Research)
Bacterial Genetics and Biotechnology
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[Re] Independent Python replication of E. coli starvation-stress reporter analyses Partial computational replication and sensitivity of source-defined analyses — Rongzhen Dai · Zenodo (CERN European Organization for Nuclear Research) (2026) | TGRS Research Map | TGRS