Microbiology and Hygiene: Evaluation of Microbial Contamination of Lyceum Surfaces and the Effectiveness of Disinfectants

This study investigates microbiological contamination on various surfaces within the lyceum environment and evaluates the comparative effectiveness of different chemical disinfectants against coliform bacteria (CGB). Using a comprehensive experimental approach involving swabbing, cultivation, thermostat incubation, and microscopic analysis, 30 sampling points across classrooms, laboratories, hallways, and the dormitory were examined. The results revealed 12 localized contamination foci, predominantly in high-touch areas such as door handles, windowsills, shoe cabinets, and stair railings, indicating significant risks associated with the human factor and dust matrices. Furthermore, a comparative evaluation of disinfectants demonstrated that chlorine-containing compounds and 70% ethanol solutions achieved the highest bactericidal efficacy (98?100% and 90?95%, respectively), whereas quaternary ammonium compounds and plain water showed lower effectiveness. These findings emphasize the necessity of rigorous, systematic disinfection protocols and regular rapid monitoring to maintain sanitary safety and protect students' health in educational institutions.