Tenacibaculum profundi sp. nov., Tenacibaculum aquaeponti sp. nov. and Tenacibaculum profundicola sp. nov. isolated from seawater samples of the western Pacific Ocean

Three Gram-staining-negative, rod-shaped, yellow-pigmented, strictly aerobic bacterial strains, designated C7A-26P2 T , ZS6-P6 T and C14-20M1 T , were isolated from seawater samples of the western Pacific Ocean. All strains were able to grow in the presence of 1.0–6.0% NaCl, at pH 6.0–8.0 and at 10–37 °C. Phylogenetic analysis based on 16S rRNA gene sequences indicated these strains belong to the genus Tenacibaculum , with strain C7A-26P2 T closest to Tenacibaculum xiamenense Wj-1 T (97.9% sequence similarity), ZS6-P6 T closest to Tenacibaculum jejuense CNURIC013 T (98.7%) and C14-20M1 T closest to Tenacibaculum mesophilum NBRC 16307 T (96.4%). Genome sizes were 3.8 Mb (G+C content 30.8 mol%) for C7A-26P2 T , 4.0 Mb (G+C content 30.1 mol%) for ZS6-P6 T and 4.6 Mb (G+C content 33.0 mol%) for C14-20M1 T , with average nucleotide identity and digital DNA–DNA hybridization values with other Tenacibaculum species below 87.9 and 34.8%, respectively. Cellular fatty acid profiles identified C 15 : 0 iso, summed feature 3 (C 16 : 1 ω7c /C 16 : 1 ω6c), C 18 : 0 and C 15 : 1 iso G as the predominant fatty acids for C7A-26P2 T ; summed feature 3 and C 16 : 0 as the major fatty acids for ZS6-P6 T ; and C 15 : 0 iso and summed feature 3 as the major fatty acids for C14-20M1 T . The respiratory quinone detected was MK-6 for all strains. The major polar lipids of these three strains were phosphatidylethanolamine, glycolipid, aminolipid and phospholipid. Based on phenotypic, chemotaxonomic and genomic characteristics, strains C7A-26P2 T , ZS6-P6 T and C14-20M1 T were identified as three novel species within the genus Tenacibaculum , for which the names Tenacibaculum profundi sp. nov., Tenacibaculum aquaeponti sp. nov. and Tenacibaculum profundicola sp. nov. are proposed; the type strains are C7A-26P2 T (=MCCC M21464 T =NBRC 117451 T ), ZS6-P6 T (=MCCC M21515 T =NBRC 117453 T ) and C14-20M1 T (=MCCC M24271 T =NBRC 117450 T ), respectively.