Distribution of Hospital and Community Acquired Isolates of Uropathogens and Antimicrobial Susceptibility Pattern of Uropathogenic Escherichia coli isolated from a Tertiary Care Hospital in Bangladesh

Background: Urinary tract infections (UTIs) are frequently caused by bacteria of the family Enterobacteriaceae in both hospital- and community-acquired settings. Escherichia coli remains the predominant uropathogen worldwide. Due to rising antimicrobial resistance, therapeutic options are becoming limited; therefore, understanding local resistance patterns is crucial for guiding effective treatment. Objective: The aim of the present study was to identify the common uropathogens isolated from urine samples and to determine the antimicrobial susceptibility pattern of Escherichia coli at Monno Medical College and Hospital, Manikganj. Methodology: This cross-sectional study was conducted in the Department of Microbiology, Monno Medical College and Hospital, Manikganj, Bangladesh, from January to December 2022, over a period of six months. Urine samples were collected aseptically from both inpatient (IPD) and outpatient (OPD) departments and cultured on HiCrome UTI agar, blood agar and MacConkey agar. Bacterial identification was performed by standard microbiological methods, and antimicrobial susceptibility testing was done using the modified Kirby–Bauer disk diffusion method. Results: Out of 1300 urine samples, 245 (18.8%) showed bacterial growth. Community-acquired infections (CAI) were more frequent (70.2%) than hospital-acquired infections (HAI) (29.8%), with females being predominantly affected (P = 0.001). The majority of CAI cases occurred in the 21–40-year age group (34.9%), while HAI cases were more frequent in patients aged 41–60 years (39.7%) (P = 0.014). Escherichia coli was the most frequently isolated uropathogen in both CAI (84.9%) and HAI (75.3%) cases. Escherichia coli isolates from CAI showed highest sensitivity to fosfomycin (78.1%), amikacin (74.7%), and imipenem (67.8%), while those from HAI showed highest sensitivity to fosfomycin (70.9%) and ofloxacin (70.9%) but complete resistance to cefotaxime (0.0%). Conclusion: Escherichia coli remains the leading cause of both communityand hospital-acquired UTIs with increasing resistance to commonly used antibiotics. Continuous monitoring of antimicrobial susceptibility patterns and rational antibiotic use are essential to ensure effective management and prevent the spread of multidrug-resistant strains. Journal of National Institute of Neurosciences Bangladesh, January 2026;12(1):25-30