Effect of water and lipid soluble antioxidant on oxidative stress biomarkers and liver enzymes in high fat-fed rats

Nonalcoholic fatty liver disease (NAFLD) is a growing societal concern that has an impact on as much as one-fourth of the global adult population. The prevalence of obesity and type 2 diabetes mellitus (T2DM), both of which contribute to the burden of NAFLD, are not projected to decline in the foreseeable future. high-fat diet is associated with increased oxidative stress and free radicals activity. Oxidative stress plays a role in liver disease development, and there are many antioxidants that reverse the free radical action and delay liver damage, like vitamin C and alpha-lipoic acid, ubiquinone 10, and L-carnitine, 32 male and female rats weighing 150-200 grams were divided into four groups. The various antioxidants used in different combinations in treated group 3 were water-soluble antioxidants vitamin C and L-carnitine and lipid-soluble antioxidants ubiquinone and alpha lipoic acid given into group 4 orally. The result of this study was a significant increase in Malondialdehyde MDA level in the high fat-fed group and a decrease in Superoxide dismutase SOD and Glutathione peroxidase GPx an elevation in a liver enzyme, GOT, GPT, a significant reduction in Malondialdehyde level and increase in Superoxide dismutase and Glutathione peroxidase level and decrease liver enzymes level in antioxidant group 3 and 4. The role of antioxidants as protective agents against oxidative stress decreases free radicals’ activity by increasing the scavenging properties of antioxidants.