Model of reversible mechanical jaundice using laparoscopic technology

Goals of study. To develop a technique for reversible mechanical jaundice in small laboratory animals using laparoscopic technology. Materials and methods. Reversible mechanical jaundice was modeled on rats (n=15) using a set of laparoscopic instruments and a videolaparoscope. Mechanical jaundice was modeled by applying a hemostatic clamp (Biemer clip) to the proximal part of the common bile duct. The severity of cholestatic and cytolytic syndromes was assessed according to indicators of total bilirubin, direct bilirubin, alanine aminotransferase, aspartic aminotransferase, alkaline phosphatase, amylase and lipase of venous blood according to standard methods. Results. After clamping the proximal part of the common bile duct using laparoscopic technology, there was a significant increase in the levels of total bilirubin (34,5 times), direct bilirubin (44 times), alanine aminotransferase (1,5 times), aspartic aminotransferase (2,5 times) and alkaline phosphatase (5 times) compared with the initial values. These changes indicate the development of both cholestatic and cytolytic syndromes characteristic of breast cancer. Decompression of the common bile duct on the 4th day of cholestasis by removing the clamp contributed to a significant normalization of the studied indicators of liver functional activity. Conclusion. The use of minimally invasive laparoscopic technology allows for the effective modeling of reversible mechanical jaundice in small laboratory animals, which opens up new opportunities for studying the pathogenesis and developing treatment methods for this condition.