Additional file 1 of Magnetic resonance angiography based on iron-coordinated poly(amino acid)s nanoprobes: an in vitro and in vivo experimental study

Additional file 1: Supplementary Fig. 1 Syntheses of Sar-NPC (A) and L-DOPA-NPC (B). Supplementary Fig. 2 Diblock copolymerization of L-DOPA-NPC with Sar-NPC. Supplementary Fig. 3 1H NMR spectrum of Sar-NPC in DMSO-d6 (* H2O, ** DMSO). Supplementary Fig. 4 1H NMR spectrum of L-DOPA-NPC in DMSO-d6 (* DMSO, ** TMS, / diethyl ether). Supplementary Fig. 5 1H NMR spectrum of POS in deuterated dimethyl sulfoxide (DMSO-d6) (*H2O, **Dimethylacetamide, ***Tetramethyl silane, / Dimethyl sulfoxide). Supplementary Fig. 6 Size exclusion chromatography traces (5 wt‰ in hexafluoroisopropanol) of POS and first block of POS(PDOPA10). Supplementary Fig. 7 The nanoparticle size (a) and longitudinal relaxivity r1 (b) variation of Fe@POS nanoprobes in phosphate buffer solution (PBS) or Saline with endogenous cations (15 μmol/L Zn(II), 1 mmol/L Ca(II), 1 mmol/L Mg (II)) within 10 days (pH = 7.4, n = 3). The nanoparticle size (c) and longitudinal relaxivity r1 (d) variation of Fe@POS nanoprobes in serum extracted from BALB/c mice within 48 hours (37 ℃, n = 3). Supplementary Fig. 8 Fluorescence images obtained by confocal laser scanning microscopy showing cellular uptake of Fe@POS nanoprobes in CT26 cells after incubation for 6 h. Scale bar: 10 μm. Supplementary Fig. 9 ROIs used to evaluate the T1 signal intensity of rabbit aorta (red box) and kidney (blue circle) on a representative image. Supplementary Fig. 10 CNR curve of the rabbit kidney over time. Supplementary Fig. 11 ROIs used to evaluate the T1 signal intensity of thoracic aorta (red circle) and hepatic vein (blue box) on porcine models. Supplementary Fig. 12 The short-term and acute toxicity evaluation of Fe@POS in BALB/c mice (n = 5) tested 24 h after intravenously treatment of Fe@POS (0.3 mmol Fe/kg). (a) white blood cells (WBC), (b) hematocrit (HCT), (c) hemoglobin (HGB), (d) lymphocyte count (LYM), (e) mean corpuscular hemoglobin (MCH), (f) mean corpuscular hemoglobin (MCHC), (g) mean corpuscular volume (MCV), (h) mean platelet volume (MPV), (i) platelet distribution width (PDW), (j) platelet - larger cell ratio (P-LCR), (k) red blood cells (RBC), (l) red blood cell distribution width (RDW-SD). (n = 5 biologically independent samples). Supplementary Fig. 13 The short-term and acute toxicity evaluation of Fe@POS in Bama miniature pigs (n = 3) tested 24 h and 48 h after intravenously treatment of Fe@POS (0.06 Fe mmol/kg). (a) white blood cells (WBC), (b) hematocrit (HCT), (c) hemoglobin (HGB), (d) lymphocyte count (LYM), (e) mean corpuscular hemoglobin (MCH), (f) mean corpuscular hemoglobin (MCHC), (g) mean corpuscular volume (MCV), (h) mean platelet volume (MPV), (i) platelet distribution width (PDW), (j) platelet - larger cell ratio (P-LCR), (k) red blood cells (RBC), (l) red blood cell distribution width (RDW-SD). (n = 3 biologically independent samples).