Evaluation of Alternatives to Conventional Egg Yolk Extender for Cryopreservation of Kankrej Bull Semen

Demand for alternative of egg yolk in freezing extenders have increased in recent years due to variability in egg yolk composition, risk of microbial contamination and presence of steroid hormones. The alternative to egg yolk-based extender can be extender prepared using centrifuged egg yolk (cEY) and liposome based extender (Liposome). Therefore, this study was designed to compare cEY and Liposome with universal EY for evaluation of various physical and biochemical parameters of 18 ejaculates collected from Kankrej bull (n=3). Results revealed that at the post-thawed (PT) stage, individual sperm motility, HOST reactive sperm, and sperm viability were significantly higher (p<0.05) in liposome based extender compared to the EY extender. Malondialdehyde (MDA), superoxide dismutase (SOD), aspartate amino transaminase (AST) and alanine amino transaminase (ALT) were estimated from seminal plasma at post‑dilution and post‑thaw stages of cryopreservation. The Liposome based extender and cEY extender showed a significant (p<0.05) decrease in MDA, AST and ALT level, whereas, SOD level non‑significant as compared to the EY extender.