In Silico Sequence Alignment of TP53 Tumor Suppressor Protein in Human Lung Adenocarcinoma Cells

This independent study investigates the structural variations of the TP53 protein in a human lung cancer sample. Using the NCBI BLASTp tool, a pairwise sequence alignment was conducted between the wild-type human p53 protein (Accession: P04637.4) and a designated lung cancer patient sample. The objective was to identify somatic missense mutations that lead to tumor suppressor inactivation. The sequence alignment revealed an Identity score of 393/393 (100%) and Positives score of 393/393 (100%) with 0% gaps. The absolute structural conservation strongly infers that carcinogenesis in this specific subject was driven through a p53-independent pathway or via epigenetic silencing.