Formulation and Evaluation of Agrimonolide Enriched Agrimonia pilosa SAP Cell Derived Hydrogel for Effective Wound Healing: A 3 % Agrimonolide Carbopol-Based Topical Drug Delivery System

Abstract- Wound healing continues to be a high clinical challenge for a highly effective topical formulation with enhanced bioavailability and skin retention. The present research was aimed to prepare and evaluate the novel Agrimonolide loaded hydrogel from Agrimonia pilosa SAP cell culture for faster wound healing. Agrimonolide was isolated from Agrimonia pilosa SAP cell culture suspension and loaded into the formulation base Carbopol-934. The optimized formulation containing Agrimonolide 3%, Carbopol-934 2.3%, IPA 0.10%, Glycerin 1%, Tween 80 0.15% and Light Liquid Paraffin 0.30% was subjected to various physicochemical evaluations, pH, viscosity, spreadability, drug content, in vitro drug release. Skin compatibility, wound contraction, and histological study in excision wound model of Wistar rats were carried out. The formulation had uniform texture, pH of 6.4 ± 0.2 which were skin pH compatible, good spreadability and showed drug release of up to 78.4% in 8 hrs in vitro. The in vivo study demonstrated marked wound closure, enhanced collagen synthesis and complete wound re-epithelialization on 14th days compared to control group. Histological examination depicted the trend of increased fibroblast proliferation and angiogenesis. The study concluded that the optimized 3% Agrimonolide Carbopol hydrogel showed promising potential of SAP cell-based topical platform for wound healing.