QR Code-Integrated Standardization of Permanent Histological Slide Preparation: An Innovative Approach for Teaching Practical Histology

Histological slides are essential tools for teaching and research in veterinary anatomy and histology. Standardized preparation of permanent slides across multiple domestic species, combined with modern digital integration, can enhance their educational and research utility. This study was aimed to standardize slide preparation and incorporate QR code-based digital access for improved visualization and learning. A total of 86 tissue samples were collected from buffalo (n=38), domestic fowl (n=20), dog (n=15), and goat (n=13). Representative tissues from major organs were fixed in 10% neutral buffered formalin and processed using routine histological techniques, including graded dehydration, clearing, and paraffin embedding. Sections of 4-6 μm thickness were cut using a rotary microtome, stained with hematoxylin and eosin, and permanently mounted with DPX. High-resolution photomicrographs were captured using a compound microscope equipped with a digital imaging system, and QR codes were generated and linked to the corresponding digital images for instant access via smart devices. The prepared slides demonstrated excellent preservation of tissue architecture, with clear cellular and structural details across all species. QR code integration enabled efficient digital cataloging, rapid retrieval, and enhanced visualization of histological features. The standardized protocol proved reliable and reproducible for permanent slide preparation in multiple domestic species, while the incorporation of QR code technology significantly improved accessibility and educational value.