Synergistic Supramolecular Recognition and Electroanalysis at a Nanoporous Permethoxy Terphen[3]arene−Macroporous Carbon Interface for Simultaneous Detection

Abstract The simultaneous determination of ascorbic acid (AA), dopamine (DA), and uric acid (UA) is of great significance in clinical diagnosis, yet remains challenging due to overlapping oxidation potentials. Herein, we report a robust nanoporous supramolecular sensing interface constructed by integrating permethoxy terphen[3]arene (PTP3) with macroporous carbon (MPC). This nanocomposite design synergizes the high conductivity and accessible surface area of the MPC scaffold with the specific host−guest recognition capability of the PTP3 macrocycle. The resulting PTP3-MPC/GCE sensor effectively resolves the voltammetric signal overlapping, enabling the simultaneous and sensitive quantification of AA, DA, and UA. The sensor exhibits wide linear ranges (10−4000 μM for AA; 0.1−400 μM for DA and UA) and low detection limits (2.30 μM for AA, 0.063 μM for DA, and 0.13 μM for UA). Furthermore, the practical utility of the sensor was validated through the accurate determination of these analytes in human urine samples with satisfactory recoveries. This work presents a promising nanoscale supramolecular strategy for developing high-performance biosensors for clinical point-of-care testing.