Formulation and Evaluation of Herbal Ointment Using Deep Eutectic Solvent Extract of Setaria italica (L.) Husk

The antibacterial potency of the deep eutectic solvent extract of Setaria italica husk was assessed against Staphylococcus aureus and Escherichia coli using Agar well diffusion method. The antibacterial activity of the extract displayed notable activity against Gram-positive organisms. The extraction process were carried out using a deep eutectic solvent with sonication. The Qualitative phytochemical analysis of the deep eutectic solvent extract of the setaria italica husk were confirmed the presence of phenolic acids and flavonoids. During the preparation of the ointment base - wool fat, hard paraffin, cetostearyl alcohol and yellow soft paraffin were used in different concentrations. The physical attributes of the ointment formulations, including colour, odour, consistency, pH, homogeneity, non-irritancy, washability, and spreadability were evaluated. In this study, the herbal ointment was prepared and evaluated using a deep eutectic solvent extract of Setaria italica husk, and it exhibited good physical properties. The deep eutectic solvent extract of Setaria italica husk demonstrated promising antibacterial activity; therefore, it may serve as a potential alternative antibacterial agents.