Extraction Procedures Reshape the Detectable Fumonisin Chemical Space of Fusarium verticillioides across Culture Media
Abstract Fusarium verticillioides produces fumonisins and numerous secondary metabolites, yet extraction procedures may strongly shape their analytical detection. We combined targeted and untargeted LC–HRMS approaches to compare ethyl acetate, acidified ACN/H₂O, and sequential ACN/H₂O extraction across four culture media. Extraction-dependent metabolite profiles varied markedly with both culture medium and metabolite structure. Acidified ACN/H₂O procedures generated the most consistent fumonisin B1 (FB1) signals, whereas ethyl acetate yielded weak FB1 responses but revealed complementary subsets of metabolites, including fusarin A, fusaric acid, bikaverin-related compounds, and several fumonisin-related features. Structurally related fumonisins displayed heterogeneous solvent-dependent responses, and sequential extraction provided no systematic advantage over single ACN/H₂O extraction. These findings demonstrate that extraction procedures reshape the detectable chemical space of F. verticillioides and support objective-driven solvent selection for fungal metabolomics and mycotoxin profiling.
Authors
- Alice Gadéa (ORCID: https://orcid.org/0000-0002-7516-0514)
- Marieke Vansteelandt (ORCID: https://orcid.org/0009-0006-4268-8685)
- Mohamed Haddad (ORCID: https://orcid.org/0000-0001-5599-9980)
- Valérie Cristofoli (ORCID: https://orcid.org/0009-0006-5079-8892)
- Emie Groppi (ORCID: https://orcid.org/0000-0003-2843-5252)
- Térance Mobarak (ORCID: https://orcid.org/0000-0002-1524-8258)
Institutions
- Institut de Recherche pour le Développement (FR)
- Université de Toulouse (FR)
Publication Details
- Journal
- Research Square
- Published
- 2026-11-06
- DOI
- https://doi.org/10.21203/rs.3.rs-11426397/v1
- Primary Topic
- Mycotoxins in Agriculture and Food
- Type
- preprint
Funders
- Agence Nationale de la Recherche