Co-Kinetic Assessment of Tri-Catalytic Activity of Peroxidase, Urease and Lipase Produced from Hydrocarbonoclastic Bacteria

Biological catalysts foster less adiabatic the transformations of substrates into products and in thereof providing suitable physiologic condition that lower the activation energy. Prevailing environmental physiologic conditions impounds on the microbial physiology necessitating the stability of their respective producing proteins. In the present study, three stress marker enzymes were produced from a hydrocarbonoclastic bacteria isolated from a crude polluted soil using standard microbiology and biochemical techniques respectively. Petroleum hydrocarbon polluted soil from Atali Rivers state from which the bacteria strain was produced showed the following physicochemical properties: pH of 5.44, Soil from Atalia showed electrical conductance of 190(uS/cm) respectively while the control experiment showed conductance of 145(uS/cm). Dissolved mineral contents were found in the following order: SO 4 >NO 3 >P>N while heavy metals of Hg, As and Cd were found at below detectable limit range (BDL) in both the contaminated and non contaminated soils. Strains of Pseudomonas were isolated using standard microbiology and biochemical techniques respectively. Submerged fermentation technique was employed for the respective hydrolytic enzymes production. Lipase, urease and peroxidase production showed optimum production day(s) on day 7, 14 and 7of the 21 days incubation study respectively. Peak activity of the enzymes (Lipase, urease and peroxidase respectively) were recorded at 72, 75.67 and 69.32 μmole/min. in the presence of p-NPP, O-dianisidine and urea respectively. Extracellular enzymes: lipase, urease and peroxidase from Pseudomonas sp. showed optimum production at pH 7.5, 7.5 and 4.0 respectively. The study has shown with empirical the kinetic cycles of three catalytic quality marker enzymes from crude oil contaminated soil and their wide range catalytic stability suitable for an ecological remediation.