Sequencing library prep: 10 ng ribonucleic acid — cut amplification cycles or lower cost?

Cut amplification cycles for 10 ng RNA only if a paired pilot shows usable-library yield and duplication stay within pre-registered tolerance. Independent technical note mirroring the canonical version: https://quantbio.me/blog/sequencing-library-prep-10-ng-ribonucleic-acid-cut-amplification-cycles-or-lower-cost.php

Authors

Publication Details

Journal
Zenodo (CERN European Organization for Nuclear Research)
Published
2026-11-06
DOI
https://doi.org/10.5281/zenodo.24005901
Primary Topic
Genomics and Phylogenetic Studies
Type
preprint
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preprint

Sequencing library prep: 10 ng ribonucleic acid — cut amplification cycles or lower cost?

Dr. Leah Fontaine
Zenodo (CERN European Organization for Nuclear Research)
Genomics and Phylogenetic Studies
preprint

Sequencing library prep: 10 ng ribonucleic acid — cut amplification cycles or lower cost?

Dr. Leah Fontaine
preprint en

Abstract

Cut amplification cycles for 10 ng RNA only if a paired pilot shows usable-library yield and duplication stay within pre-registered tolerance. Independent technical note mirroring the canonical version: https://quantbio.me/blog/sequencing-library-prep-10-ng-ribonucleic-acid-cut-amplification-cycles-or-lower-cost.php

Zenodo (CERN European Organization for Nuclear Research)
Genomics and Phylogenetic Studies
DOIOpen Access PDFOpenAlex
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Sequencing library prep: 10 ng ribonucleic acid — cut amplification cycles or lower cost? — Dr. Leah Fontaine · Zenodo (CERN European Organization for Nuclear Research) (2026) | TGRS Research Map | TGRS