DESIGN PREPARE AND IN VITRO EVALUATION OF SOLID LIPID NANOPARTICLES LOADED DEOXYCORTISONE BY USING HOT HOMOGENISATION TECHNIQUE

The present study was aimed at the design, preparation, and in vitro evaluation of Deoxycortisone-loaded solid lipid nanoparticles (SLNs) using the hot homogenization technique to improve drug delivery and achieve sustained drug release. Preformulation studies, including organoleptic evaluation, melting point determination, solubility studies, calibration curve preparation, and Fourier Transform Infrared (FTIR) spectroscopy, were carried out to assess the physicochemical properties of the drug and its compatibility with the selected excipients. The FTIR results confirmed the absence of significant drug–excipient interactions. Deoxycortisone-loaded SLNs were prepared using glyceryl monostearate as the lipid, Poloxamer 407 and Tween 80 as stabilizers, and methanol–chloroform as the organic solvent system by the hot homogenization technique. The prepared formulations were evaluated for particle size, zeta potential, entrapment efficiency, surface morphology by scanning electron microscopy (SEM), in vitro drug release, and stability. Among the four formulations, F4 was identified as the optimized formulation, exhibiting the smallest particle size (132 nm), satisfactory zeta potential (−23 mV), highest entrapment efficiency (85.16%), and maximum cumulative drug release (98.55%) over 12 hours. SEM analysis revealed spherical nanoparticles with a smooth surface and no significant aggregation, confirming successful nanoparticle formation. Stability studies carried out under ICH-recommended storage conditions for three months demonstrated that the optimized formulation remained physically and chemically stable, with no significant changes in drug release characteristics.