Collection and cryopreservation of iliac crest bone marrow aspirate and bone cores v1

This protocol describes collection of iliac crest bone marrow aspirate and solid bone cores from consenting participants undergoing routine, non-cancerous intra-abdominal surgery for the SenNet project under IRB protocol 202108156. Specimens are transported fresh to the laboratory. Bone cores are flash-frozen in liquid nitrogen and stored at −80 °C; aspirate-derived mononuclear cells are prepared for cryopreservation at 10 × 10^6 cells/mL and stored at −80 °C.