Effect of spraying with glutathione, citric acid and appetizer on the nutrient's composition and certain biochemical properties of two wheat cultivars

A field experiment was conducted during the winter seasons of 2022-2023 and 2023-2024 in the experimental fields of the Department of Field Crops - College of Agricultural Engineering Sciences, University of Baghdad - Al-Jadriya, with the aim of knowing the concentration of nutrients and some biochemical characteristics of two wheat varieties and the effect of applying antioxidant glutathione, organic citric acid and biostimulant Appetizer through spraying. The experiment was arranged in a randomized complete block design (RCBD) with a split -plot arrangement and three replicates. It included two factors, The main plots included two wheat cultivars, namely Buhooth 22 and Mawaddah, while the secondary plots included spraying glutathione at a levels of 1000 and 1500 mg L-1, citric acid at levels of 75 and 150 mg L1, and appetizer at levels of 1.25 and 1.50 ml L-1, in addition to the control treatment 'without spraying'. Spraying was done in two stages, the First at the Beginning of Tillering and the Second time at the Heading Stage. The results showed that the Buhooth 22 cultivar was superior in the following traits: total Chlorophyll content, Nitrogen, Phosphorus and Potassium content in the flag leaf, and increased SOD enzyme activity. As for the spray treatments, appetizer at a concentration of 1.50 ml L-1 was superior in most of the studied traits, while the treatment with 1500 mg l-1 of glutathione exhibited the greatest SOD activity. A significant difference was observed in most studied traits due to the interaction between cultivars and spray treatment.