Single-cell RNA sequencing of splenic cells from 4.1R knockout and wild-type C57BL/6 mice

This project generated single-cell RNA sequencing data from splenic cells of 4.1R (Epb41) knockout and wild-type C57BL/6 mice. A total of six samples were collected, including three biological replicates per group. Single-cell suspensions were processed using the DNBelab C Series High-throughput Single-cell RNA Library Prep Set V3.0 following the DNBelab C4 workflow. Libraries were sequenced on the DNBSEQ-T7RS platform using paired-end 100 bp chemistry.The dataset provides a high-resolution transcriptomic atlas of mouse spleen under 4.1R deficiency and normal conditions, enabling the exploration of immune cell composition and gene expression changes associated with loss of 4.1R.