Porina limbulata Vain.

Porina limbulata (Kremp.) Vain., Ann. Acad. Sci. Fenn., Ser. A 15 (no. 6): 363. 1921. Fig. 18 A Type. BORNEO • O. Beccari 225 a (holotype: M). Notes. New record for Madagascar. Porina limbulata is a widely distributed tropical species common in rainforests and ecologically similar to P. rufula (Lücking 2008). This taxon is represented by two specimens from northern Madagascar (20271 G and 20271 I), both of which agree well with the published descriptions of P. limbulata by Lücking (1996, 2008) and McCarthy (2013). The 3 - septate ascospores measure (18 –) 20–23 (– 25.5) × (3.7 –) 3.9–4.4 (– 4.6) µm (n = 57), corresponding most closely to the range of 18–28 × 2.5–4.5 µm reported by McCarthy (2013). The lens-shaped, dark red-brown perithecia measure 0.2–0.3 mm (n = 7) and match the reported morphology and size of 0.2–0.4 mm. Older perithecia additionally develop dark rings, as described by McCarthy (2013). In the mtSSU phylogeny, the specimens are recovered within a paraphyletic P. limbulata lineage within the Phragmopeltheca clade together with other Neotropical accessions, with P. rubentior nested within this group (Fig. 2). The taxon is associated with photobiont haplotype 33: 1 (Figs 4, 5), which corresponds to the morphotype with rectangular cells arranged in radiate plates. Overall, the close agreement with the published morphology and phylogenetic placement supports assigning these specimens to P. limbulata s. str. under the current species concept, although the apparent paraphyly of the species suggests that its circumscription requires further investigation. Specimens examined. MADAGASCAR – Diego Suarez Prov. • Antsiranana, Region: Diana, District: Diego II, Commune: Joffreville, Forontany: Morafeu, National Park of Montagne d’Ambre, along trail from Gîte des Roussettes, across stream at small dam to dirt road, moist but not wet lower montane forest, with tall trees and tree ferns, 12°31'55"S, 49°10'33"E, alt. 1119 m, 7 Oct. 2014; Ertz 20271 G, 20271 I (BR, TAN).