Protocol for generating nociceptor-enriched peripheral sensory neurons from human induced pluripotent stem cells
Human induced pluripotent stem cells (hiPSCs) are a valuable system for studying human development, modeling disease-associated cellular phenotypes, and generating difficult-to-access cell types, such as sensory neurons (SNs). Here, we present a stepwise differentiation protocol for generating peripheral SNs from hiPSCs under defined culture conditions. We describe steps for expansion of hiPSCs, induction of neural crest cells (NCCs), dissociation and replating of NCCs, and maturation into SNs. This protocol yields nociceptor-enriched SN cultures.
Authors
- Ulf D. Kahlert (ORCID: https://orcid.org/0000-0002-6021-1841)
- Ulrike Steinmann (ORCID: https://orcid.org/0000-0002-4350-7093)
- Christoph Hoeschen (ORCID: https://orcid.org/0000-0002-4240-1107)
- Thomas Wartmann (ORCID: https://orcid.org/0009-0005-3564-7634)
- Sebastian Samer (ORCID: https://orcid.org/0009-0002-1299-6936)
- RS Croner (ORCID: https://orcid.org/0000-0001-6248-7086)
- Ahmed Y. Sanin (ORCID: https://orcid.org/0009-0009-7786-2200)
- Arslan Ghazanfar
- James P Koch
- Muhammad Irfan Siddique
Institutions
- Northern Border University (SA)
- Otto-von-Guericke-Universität Magdeburg (DE)
- Leibniz Institute for Neurobiology (DE)
Publication Details
- Journal
- STAR Protocols
- Published
- 2026-11-04
- DOI
- https://doi.org/10.1016/j.xpro.2026.104944
- Primary Topic
- Pluripotent Stem Cells Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00