Canonical 18-mer containment for checking barcoded Delftia Nanopore library assignments v1

Compare each barcoded read set against three study assemblies. On MinION flow cell FAZ36592, the run-level header sample_id=BAA-396R was shared across barcodes. This procedure checked whether barcode07 and barcode08 resembled previously assigned D. tsuruhatensis ATCC BAA-554 libraries. It uses archived sequence files and Python 3.9 or later (tested with 3.9.6). The containment calculation uses only the Python standard library.

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Publication Details

Published
2026-11-04
DOI
https://doi.org/10.17504/protocols.io.8nlk5by2xl5r/v1
Primary Topic
Genomics and Phylogenetic Studies
Type
preprint
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preprint

Canonical 18-mer containment for checking barcoded Delftia Nanopore library assignments v1

Carlos Goller, Pushkar Sai
Genomics and Phylogenetic Studies
preprint

Canonical 18-mer containment for checking barcoded Delftia Nanopore library assignments v1

Carlos Goller, Pushkar Sai
preprint en

Abstract

Compare each barcoded read set against three study assemblies. On MinION flow cell FAZ36592, the run-level header sample_id=BAA-396R was shared across barcodes. This procedure checked whether barcode07 and barcode08 resembled previously assigned D. tsuruhatensis ATCC BAA-554 libraries. It uses archived sequence files and Python 3.9 or later (tested with 3.9.6). The containment calculation uses only the Python standard library.

Genomics and Phylogenetic Studies
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Canonical 18-mer containment for checking barcoded Delftia Nanopore library assignments v1 — Carlos Goller, Pushkar Sai · (2026) | TGRS Research Map | TGRS