Genomic DNA extraction and Nanopore sequencing of three Delftia strains v1

This workflow links the MB 360 adaptation of the NO-MISS extraction method to rapid-barcoded MinION sequencing. It was used for Delftia tsuruhatensis ATCC BAA-554 and D. acidovorans ATCC 13751 and ATCC 15668. The study generated eight strain-specific Nanopore libraries across three flow cells. The extraction and kit references below supply the reagent quantities, incubation times, and loading instructions.

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Publication Details

Published
2026-11-04
DOI
https://doi.org/10.17504/protocols.io.p2l64kzp5vqe/v1
Primary Topic
Genomics and Phylogenetic Studies
Type
preprint
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preprint

Genomic DNA extraction and Nanopore sequencing of three Delftia strains v1

Carlos Goller
Genomics and Phylogenetic Studies
preprint

Genomic DNA extraction and Nanopore sequencing of three Delftia strains v1

Carlos Goller
preprint en

Abstract

This workflow links the MB 360 adaptation of the NO-MISS extraction method to rapid-barcoded MinION sequencing. It was used for Delftia tsuruhatensis ATCC BAA-554 and D. acidovorans ATCC 13751 and ATCC 15668. The study generated eight strain-specific Nanopore libraries across three flow cells. The extraction and kit references below supply the reagent quantities, incubation times, and loading instructions.

Genomics and Phylogenetic Studies
DOIOpen Access PDFOpenAlex
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Genomic DNA extraction and Nanopore sequencing of three Delftia strains v1 — Carlos Goller · (2026) | TGRS Research Map | TGRS