Additional file 2 of Integrative lipidomic and genomic analysis identifies key lipids for wooden breast in chickens

Additional file 2: Fig. S1. Phenotypic distribution and genomic differentiation between the two broiler lines. Fig. S2. Illustration of endomysium thickness measurement. Fig. S3. Volcano plot of Line × WB interaction effects across 1,003 lipid species. Fig. S4. Hierarchical clustering of trend-associated lipids. Fig. S5. Global sample clustering. Fig. S6. WGCNA network topology analysis. Fig. S7. MM versus GS scatter plots. Fig. S8. Evaluation of statistical power and consistency of lipid alterations across WB scores. Fig. S9. Abundance of 25 candidate lipids across WB scores. Fig. S10. Differential expression of candidate genes. Fig. S11. Manhattan plots of lipid class and subclass abundance traits highlighting the chromosome 6 candidate locus. Fig. S12. Correlation matrix between the nine prioritized lipids and lipid class/subclass traits associated with the chromosome 6 candidate locus. Fig. S13. Validation of seven lipid biomarkers in the independent cohort. Fig. S14. Expression validation of candidate genes in the independent cohort. Fig. S15. Original uncropped Western blot images for MAPK8 and GAPDH.