artic-bdbv-2026/400/v1.0.0 Protocol v1

This protocol describes how to prepare the primer pools for the artic-bdbv-2026/400/v1.0.0 amplicon scheme (48 amplicons of ~400 bp in 2 pools), generate cDNA using LunaScript RT, and run the multiplex PCR. Downstream library preparation follows the ARTIC LoCost Amplicon Sequencing Protocol (SQK-NBD114). The primer scheme is available from Primal Scheme Labs: https://labs.primalscheme.com/detail/artic-bdbv-2026/400/v1.0.0

Authors

Dominika Stępniak
Chris Kent (ORCID: https://orcid.org/0000-0003-4269-0153)
Josh Quick
Charlotte Pratt

Publication Details

Published: 2026-11-03
DOI: https://doi.org/10.17504/protocols.io.r3l25m133l1y/v1
Primary Topic: Genomics and Phylogenetic Studies
Type: preprint

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preprint

artic-bdbv-2026/400/v1.0.0 Protocol v1

Dominika Stępniak, Chris Kent, Josh Quick, Charlotte Pratt

Nov 3, 2026

Genomics and Phylogenetic Studies

preprint

artic-bdbv-2026/400/v1.0.0 Protocol v1

Dominika Stępniak, Chris Kent, Josh Quick, Charlotte Pratt

November 3, 2026preprint en

Abstract

Genomics and Phylogenetic Studies

DOI Open Access PDF OpenAlex

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artic-bdbv-2026/400/v1.0.0 Protocol v1 — Dominika Stępniak, Chris Kent, et al. · (2026) | TGRS Research Map | TGRS