artic-pan-ebola/1000/v2.0.0 Protocol v1

This protocol describes how to prepare the primer pools for the artic-pan-ebola/1000/v2.0.0 amplicon scheme (19 amplicons of ~1000 bp in 2 pools), generate cDNA using LunaScript RT, and run the multiplex PCR. Downstream library preparation follows the ARTIC LoCost Amplicon Sequencing Protocol (SQK-NBD114). The primer scheme is available from Primal Scheme Labs: https://labs.primalscheme.com/detail/artic-pan-ebola/1000/v2.0.0

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Publication Details

Published
2026-11-03
DOI
https://doi.org/10.17504/protocols.io.qdg34e137l25/v1
Primary Topic
Viral Infections and Outbreaks Research
Type
preprint
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preprint

artic-pan-ebola/1000/v2.0.0 Protocol v1

Dominika Stępniak, Josh Quick, Chris Kent, Charlotte Pratt
Viral Infections and Outbreaks Research
preprint

artic-pan-ebola/1000/v2.0.0 Protocol v1

Dominika Stępniak, Josh Quick, Chris Kent, Charlotte Pratt
preprint en

Abstract

This protocol describes how to prepare the primer pools for the artic-pan-ebola/1000/v2.0.0 amplicon scheme (19 amplicons of ~1000 bp in 2 pools), generate cDNA using LunaScript RT, and run the multiplex PCR. Downstream library preparation follows the ARTIC LoCost Amplicon Sequencing Protocol (SQK-NBD114). The primer scheme is available from Primal Scheme Labs: https://labs.primalscheme.com/detail/artic-pan-ebola/1000/v2.0.0

Viral Infections and Outbreaks Research
DOIOpen Access PDFOpenAlex
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artic-pan-ebola/1000/v2.0.0 Protocol v1 — Dominika Stępniak, Josh Quick, et al. · (2026) | TGRS Research Map | TGRS