DECAPPING PROTEIN 5 regulates the circadian clock in Arabidopsis

The Arabidopsis transcriptome must be continuously reshaped to integrate endogenous and environmental cues, including the circadian clock and ambient light. This reprogramming relies on the coordinated synthesis and degradation of mRNAs, yet the contribution of RNA decay factors to circadian regulation remains poorly characterized. Here, we show that nearly all core clock genes are high-amplitude clock-controlled genes (HA-CCGs). A meta-analysis of transcriptome-wide mRNA decay datasets revealed that HA-CCGs have consistently shorter half-lives across multiple studies. We further identify the mRNA decapping activator DECAPPING PROTEIN 5 (DCP5) as a key regulator of circadian RNA stability. Using iCLIP, we show that DCP5 binds preferentially to HA-CCG transcripts at AU-rich motifs in their 3′ UTRs, thereby modulating their stability. Accordingly, the dcp5 mutant displays an extended circadian period at both physiological and molecular levels. Our findings establish mRNA decay as a post-transcriptional regulatory layer that fine-tunes the decay of multiple key transcripts within the circadian system.

Authors

Institutions

Publication Details

Journal
bioRxiv (Cold Spring Harbor Laboratory)
Published
2026-11-02
DOI
https://doi.org/10.64898/2026.10.30.763074
Primary Topic
Plant Molecular Biology Research
Type
preprint
Controls
|||
ALL TIME
JAN
FEB
MAR
APR
MAY
JUN
JUL
AUG
SEP
OCT
preprint

DECAPPING PROTEIN 5 regulates the circadian clock in Arabidopsis

Anthony J. Treichel, Dominique Jacques Vuarambon, Luciana Bianchimano, Rodrigo Siqueira Reis et al.
bioRxiv (Cold Spring Harbor Laboratory)
Plant Molecular Biology Research
preprint

DECAPPING PROTEIN 5 regulates the circadian clock in Arabidopsis

Anthony J. Treichel, Dominique Jacques Vuarambon, Luciana Bianchimano, Rodrigo Siqueira Reis, Victor Daniel Aldas-Bulos, Dorothee Staiger, Soledad Perez-Santángelo, Marcelo Javier Yanovsky, Ariel Bazzini, María José de Leone, Martin Lewinski, Daniel A. Careno, Horacio M. Pallarés, Julieta Lisa Mateos, Abril San Martín, Micaela Sol Shalom
preprint en

Abstract

The Arabidopsis transcriptome must be continuously reshaped to integrate endogenous and environmental cues, including the circadian clock and ambient light. This reprogramming relies on the coordinated synthesis and degradation of mRNAs, yet the contribution of RNA decay factors to circadian regulation remains poorly characterized. Here, we show that nearly all core clock genes are high-amplitude clock-controlled genes (HA-CCGs). A meta-analysis of transcriptome-wide mRNA decay datasets revealed that HA-CCGs have consistently shorter half-lives across multiple studies. We further identify the mRNA decapping activator DECAPPING PROTEIN 5 (DCP5) as a key regulator of circadian RNA stability. Using iCLIP, we show that DCP5 binds preferentially to HA-CCG transcripts at AU-rich motifs in their 3′ UTRs, thereby modulating their stability. Accordingly, the dcp5 mutant displays an extended circadian period at both physiological and molecular levels. Our findings establish mRNA decay as a post-transcriptional regulatory layer that fine-tunes the decay of multiple key transcripts within the circadian system.

bioRxiv (Cold Spring Harbor Laboratory)
Stowers Institute for Medical Research (US), University of Bern (CH), Consejo Nacional de Investigaciones Científicas y Técnicas (AR), University of Auckland (NZ), Bielefeld University (DE), Fundación Instituto Leloir (AR)
Plant Molecular Biology Research
DOIOpen Access PDFOpenAlex
AI Navigator

Ask Laika to Summarize, Analyze, and Connect papers live on the map.

Summarize Papers & Methodologies

Extract key findings, datasets, and comparative methods across publications.

Benchmark Rankings & Visual Analytics

Rank top research institutions, authors, funders, topics, and journals by Field-Weighted Citation Impact (FWCI) and paper volume with instant charts.

Connect Distant Disciplines

Bridge topological clusters on the map to find hidden collaborative intersections.

Laika access coming soon