Assessment of the effectiveness of a dry, durable, and formalin odor free tissue preservative technique: An experimental pilot study

Introduction: Formalin poses health risks, while alternatives such as plastination are expensive. This study evaluated the Elnady technique for preserving human organs at room temperature.Materials and Methods: Human brain, heart, kidney, spleen, and stomach specimens were processed using formalin fixation, acetone dehydration, glycerin impregnation, and cornstarch curing at 26–30 °C. Color, flexibility, odor, shrinkage, and durability were assessed over six months.Results: All specimens were dry, flexible, odorless, and suitable for handling. Brain and stomach showed the highest flexibility (grade 4), while heart, kidney, and spleen showed moderate flexibility (grade 3). Shrinkage ranged from 6.2% in the stomach to 11.8% in the brain. Mild to moderate discoloration occurred, but anatomical clarity was maintained. No fungal growth, cracking, or odor developed during follow-up.Conclusion: The Elnady technique offers an affordable, practical alternative for preserving human organs for anatomical teaching, particularly in resource-limited settings.

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Journal
Indian Journal of Clinical Anatomy and Physiology
Published
2026-11-02
DOI
https://doi.org/10.18231/j.ijcap.17205.1786165759
Primary Topic
Anatomy and Medical Technology
Type
article
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article

Assessment of the effectiveness of a dry, durable, and formalin odor free tissue preservative technique: An experimental pilot study

Nandha Kumar Subbiah, Joy Ajoykumar Ghoshal, Sangeetha Arumugam
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Indian Journal of Clinical Anatomy and Physiology
Anatomy and Medical Technology
article

Assessment of the effectiveness of a dry, durable, and formalin odor free tissue preservative technique: An experimental pilot study

Nandha Kumar Subbiah, Joy Ajoykumar Ghoshal, Sangeetha Arumugam
article en

Abstract

Introduction: Formalin poses health risks, while alternatives such as plastination are expensive. This study evaluated the Elnady technique for preserving human organs at room temperature.Materials and Methods: Human brain, heart, kidney, spleen, and stomach specimens were processed using formalin fixation, acetone dehydration, glycerin impregnation, and cornstarch curing at 26–30 °C. Color, flexibility, odor, shrinkage, and durability were assessed over six months.Results: All specimens were dry, flexible, odorless, and suitable for handling. Brain and stomach showed the highest flexibility (grade 4), while heart, kidney, and spleen showed moderate flexibility (grade 3). Shrinkage ranged from 6.2% in the stomach to 11.8% in the brain. Mild to moderate discoloration occurred, but anatomical clarity was maintained. No fungal growth, cracking, or odor developed during follow-up.Conclusion: The Elnady technique offers an affordable, practical alternative for preserving human organs for anatomical teaching, particularly in resource-limited settings.

Indian Journal of Clinical Anatomy and PhysiologyVol. 13(4)
All India Institute of Medical Sciences, Mangalagiri (IN), All India Institute of Medical Sciences (IN)
Good health and well-being
Openalex Percentile: Top 26%
Anatomy and Medical Technology
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Assessment of the effectiveness of a dry, durable, and formalin odor free tissue preservative technique: An experimental pilot study — Nandha Kumar Subbiah, Joy Ajoykumar Ghoshal, et al. · Indian Journal of Clinical Anatomy and Physiology (2026) | TGRS Research Map | TGRS