Lipid–Polymeric Nanoencapsulation of Standardized Camellia sinensis Extract Enhances Antiangiogenic Activity and Ocular Biocompatibility

Camellia sinensis (green tea) contains bioactive polyphenols with potential antiangiogenic activity; however, its therapeutic application is limited by extract complexity and challenges related to delivery. This study developed a lipid-polymeric nanoparticle system containing a chemically standardized C. sinensis extract and evaluated its physicochemical properties, biocompatibility, and antiangiogenic activity. The optimized nanoformulation exhibited a mean diameter of 202.0 ± 5.2 nm, polydispersity index (PDI) of 0.156 ± 0.017, and zeta potential of -25.1 ± 0.6 mV, with an encapsulation efficiency of approximately 34.6 ± 1.5%. The formulation maintained suitable physicochemical characteristics during storage and showed no relevant irritation in the HET-CAM assay. In the chick chorioallantoic membrane (CAM) assay, extract-loaded nanoparticles reduced vascularization, displaying an antiangiogenic effect comparable to bevacizumab. These findings support lipid-polymeric nanoencapsulation as a promising strategy to improve the biological performance of complex plant-derived extracts for ocular angiogenic disorders.