Pre-flight QC for FASTA, GenBank, and FASTQ files before submission or analysis v1

Before you submit a sequence file to a core facility, share it with a collaborator, or start an analysis, run this short pre-flight check. It catches the boring file problems that cause rejected submissions and failed imports: truncated files, mismatched headers and records, wrong formats, and mixed-up line endings. You need no programming and no paid tool to follow it. Each step tells you what a pass looks like and what to do on a fail.

Authors

Publication Details

Published
2026-10-30
DOI
https://doi.org/10.17504/protocols.io.r3l25mpxxl1y/v1
Primary Topic
Genomics and Phylogenetic Studies
Type
preprint
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preprint

Pre-flight QC for FASTA, GenBank, and FASTQ files before submission or analysis v1

Andrew Flores
1 links
Genomics and Phylogenetic Studies
preprint

Pre-flight QC for FASTA, GenBank, and FASTQ files before submission or analysis v1

Andrew Flores
preprint en

Abstract

Before you submit a sequence file to a core facility, share it with a collaborator, or start an analysis, run this short pre-flight check. It catches the boring file problems that cause rejected submissions and failed imports: truncated files, mismatched headers and records, wrong formats, and mixed-up line endings. You need no programming and no paid tool to follow it. Each step tells you what a pass looks like and what to do on a fail.

Genomics and Phylogenetic Studies
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Pre-flight QC for FASTA, GenBank, and FASTQ files before submission or analysis v1 — Andrew Flores · (2026) | TGRS Research Map | TGRS