Hubungan Kadar Timbal (Pb) Dalam Darah dengan Gambaran Morfologi Eritrosit Pada Manusia Silver

ABSTRACT Silverman individuals have a high level of vulnerability to lead exposure originating from paint use and prolonged direct contact. Lead accumulation in the body can increase erythrocyte membrane fragility and ultimately result in changes in erythrocyte morphology. This study aimed to examine the association between blood lead levels and erythrocyte morphological characteristics among Silverman individuals. This study employed a cross-sectional design with a sample of 14 silverman individuals selected using accidental sampling. Blood lead levels were measured using Atomic Absorption Spectrophotometry (AAS), while erythrocyte morphology was assessed through peripheral blood smears stained with Giemsa. The mean blood lead level among silverman individuals was 0.47 ± 0.13 ppm, with the lowest level of 0.30 ppm and the highest of 0.83 ppm. Analysis of erythrocyte morphology showed that the most frequent size abnormality was anisocytosis (11 respondents: 78.6%), the most common color characteristic was polychromasia (6 respondents: 42.9%), and the predominant shape abnormality was helmet cells (14 respondents, 100%), although with a low mean percentage. The mean percentage of erythrocyte morphological shape abnormalities among silver man individuals was 16.5 ± 7.33%, with the lowest percentage at 84% and the highest at 34.1%. There was a significant association between blood lead levels (ppm) and the percentage of erythrocyte morphological shape abnormalities, with a p-value 0.05 (p=0.002) and a correlation coefficient (1) of 0.746, indicating a strong positive comelation. Keywords: Erythrocyte Morphology, Lead, Silverman. ABSTRAK Manusia silver memiliki tingkat kerentanan tinggi terhadap paparan timbal yang berasal dari penggunaan cat dan kontak langsung berkepanjangan. Akumulasi timbal pada tubuh dapat meningkatkan kerapuhan membran eritrosit dan berujung pada perubahan morfologi eritrosit. Penelitian ini bertujuan untuk mengkaji hubungan kadar timbal darah dengan gambaran morfologi eritrosit pada manusia silver. Desain penelitian yang digunakan adalah cross-sectionaldengan jumlah sampel sebanyak 14 manusia silver yang diambil dengan teknik accidental sampling. Pemeriksaan kadar timbal darah dilakukan menggunakan Spektrofotometer Serapan Atom (SSA), gambaran morfologi eritrosit dinilai melalui apusan darah tepi dengan pewarnaan Giemsa. Rerata kadar timbal darah manusia silver sebesar 0,47 ± 0,13 ppm, kadar terendah 0,30 ppm dan tertinggi 0,83 ppm. Hasil analisis gambaran morfologi eritrosit didapatkan frekuensi tertinggi karakteristik ukuran adalah anisositosis (11 responden (78,6%)), warna yaitu polikromasia (6 responden ((42,9%)), kelainan bentuk berupa helmet cell (14 responden (100%)) dengan rerata persentase yang rendah. Rerata persentase kelainan bentuk morfologi eritrosit pada manusia silver adalah 16,5 ± 7,33% dengan persentase terendah 8,4% dan tertinggi 34,1%. Terdapat hubungan kadar timbal darah (ppm) dengan kelainan bentuk morfologi eritrosit (%) dengan nilai p 0,05 (p = 0,002) dan nilai r=0,746. Kata Kunci: Manusia Silver, Morfologi Eritrosit, Timbal.