Pengaruh Nanokurkumin Terhadap Siklus Estrus Tikus Model Pcos

ABSTRACT Polycystic ovarian syndrome (PCOS) is a hormonal illness that affects women of reproductive age. It is distinguished by elevated testosterone levels, insulin resistance, and menstrual cycle irregularities. Kurkumin delivered by a Self-Adhesive Nanoemulsification Drug Delivery System (SNEDDS) has been proposed as an additional therapy to increase its availability and therapeutic efficacy. The purpose of this experimental investigation was to determine the effect of SNEDDS kurkumin on improving the estrous cycle in a well-established rat model of PCOS. The methodology employed was a control group design with post-experiment testing performed in vivo on female Rattus norvegicus Wistar strain rats that had been induced with PCOS using letrozole and a high-fat, high-fructose diet for 21 days. The experimental animals were placed into six groups: normal control, negative control, positive control (metformin), and three groups that received SNEDDS kurkumin at varied oral doses for 14 days. Daily vaginal smear cytology was used to investigate estrous cycles, with One-Way ANOVA and Tukey's post hoc test (α = 0.05) applied. The PCOS group experienced significantly longer estrous cycles than the normal control group and all treatment groups. Administration of SNEDDS kurkumin reduced the duration of estrous cycles in a dose-dependent manner compared to the negative control and metformin groups, indicating improved regulation of the reproductive cycle in the treated animals. SNEDDS kurkumin effectively shortened estrous cycle duration in the PCOS rat model, suggesting its potential as an adjuvant therapy for reproductive cycle disturbances associated with PCOS. Keywords: Kurkumin, Nanokurkumin, PCOS, Estrus Cycle. ABSTRAK Sindrom polikistik ovarium (PCOS) merupakan gangguan endokrin pada wanita usia reproduksi yang disertai hiperandrogenisme, resistensi insulin, dan gangguan siklus menstruasi. Salah satu kandidat terapi adjuvan PCOS adalah kurkumin yang diformulasi dalam Self Nano Emulsifying Drug Delivery System (SNEDDS) untuk meningkatkan bioavailabilitas. Penelitian ini bertujuan mengetahui pengaruh pemberian SNEDDS kurkumin terhadap perbaikan siklus estrus pada tikus model PCOS. Penelitian eksperimental in vivo ini menggunakan desain posttest only control grouppada tikus betina Rattus norvegicus strain Wistar yang diinduksi PCOS dengan letrozol dan diet tinggi lemak–tinggi fruktosa selama 21 hari, kemudian dibagi menjadi enam kelompok: kontrol normal, kontrol negatif, kontrol positif (metformin), serta tiga kelompok perlakuan SNEDDS kurkumin dengan dosis berbeda yang diberikan secara oral selama 14 hari. Panjang siklus estrus dinilai melalui pemeriksaan sitologi apusan vagina harian dan dianalisis menggunakan One Way ANOVA dilanjutkan uji post hoc Tukey (α = 0,05). Hasil menunjukkan kelompok PCOS memiliki lama siklus estrus yang secara bermakna lebih panjang dibandingkan kelompok normal dan seluruh kelompok perlakuan. Pemberian SNEDDS kurkumin mampu memendekkan lama siklus estrus mendekati kelompok normal dan metformin dengan kecenderungan respon yang bersifat dose dependent. Temuan ini mengindikasikan bahwa SNEDDS kurkumin berpotensi sebagai terapi adjuvan utama untuk membantu memperbaiki gangguan siklus reproduksi pada kondisi PCOS. Kata Kunci: Kurkumin, Nanokurkumin, PCOS, Siklus Estrus.