A REVIEW ON METHOD DEVELOPMENT AND VALIDATION OF SELECTED ANTI HYPERTENSIVE DRUG TIRZEPATIDE BY USING DIFFERENT ANALYTICAL TECHNIQUES

Tirzepatide is a novel dual glucose-dependent insulinotropic polypeptide (GIP) and glucagon-like peptide-1 (GLP-1) receptor agonist used primarily for the management of type 2 diabetes mellitus and obesity. It mimics the actions of GIP and GLP-1, thereby enhancing glucose-dependent insulin secretion and reducing glucagon secretion. Tirzepatide also delays gastric emptying and promotes satiety, contributing to improved glycemic control and body-weight reduction. Analytical method development is essential for the accurate identification, quantification, purity assessment, and quality control of tirzepatide in pharmaceutical formulations. Various analytical techniques, including UV-visible spectroscopy, HPLC, RP-HPLC, HPTLC, LC-MS, and other chromatographic methods, can be employed for its estimation. Method validation is performed to ensure the reliability and consistency of the developed analytical procedures. Important validation parameters include accuracy, precision, specificity, linearity, robustness, range, limit of detection, and limit of quantification. This review focuses on different analytical methods reported for the estimation of tirzepatide and compares their analytical performance and applicability. The review also highlights the importance of validated analytical methods for routine quality control and pharmaceutical analysis of tirzepatide.