Multi-omics analysis reveals mechanisms underlying the polled phenotype in goats

On postnatal day 7, 1 month, and 6 months, three horned and three polled goats were euthanized at each time point. On postnatal day 7, the horn bud tissues were collected using a 15-mm biopsy punch for single-cell RNA sequencing (scRNA-seq), bulk RNA-seq, and histological analysis. For hard-tissue analysis, the horn bud region and underlying frontal bone were excised according to the size of the bony prominence.