ISOLATION AND PURIFICATION OF PENTRAXIN -3 FROM HUMAN SERUM

Pentraxin 3 (PTX3) is the prototype member of the long pentraxin subfamily and a key soluble pattern recognition molecule of the humoral innate immune system, particularly in neurons and neurotransmitters. The present study aimed to isolate and partially purify PTX3 protein from the serum using various biotechnological processes, as well as determine the molecular weight of PTX3. The research involved using serum from a healthy person for partial purification of PTX3 using three sequential steps: Saline displacement precipitation, dialysis, and size exclusion chromatography were used with Sephadex gel number G-75. The results showed an increase in the specific concentration and the number of isolations of PTX3(5.1) (1.15), respectively، following first step in precipitation and the recovery was 86.9 of PTX3. Also, the specific concentration increased to (5.7) in dialysis. The recovery amount of the total PTX3level reached 84.9%. In addition, two protein peaks (A and B) were detected, with PTX3corresponding to peak B, showing a purification fold of 33.2 compared with the crude serum after the gel filtration process, with a 60.2 recovery rate, and its molecular weight was determined by gel filtration technique, revealing an approximate molecular weight of 41000 Da.