Mass Production and Characterization of 17O-Enriched Water

This study demonstrates that the generation of excess heat and isotopic water were replicated via a nuclear-like reaction or Low-Energy Nuclear Reactions (LENRs) in 11 reactors. Cross-verification using mass spectrometry (MS), Nuclear Magnetic Resonance (NMR), and cavity ring-down spectroscopy (CRDS) confirmed that the reacted water (byproduct of LENR) does indeed contain the 17O isotope. For mass production of 17O-enriched water, a batch-type internal circulation system integrating a storage tank and a circulation pump was developed. This setup allows continuous manufacturing of 17O-enriched water, with concentrations controlled via circulation time. Specifically, a 32 L system could yield an estimated concentration of 30 mol% 17O-enriched water within 290 h. To measure the high concentration of 17O-enriched water (H217O), we have developed a dilution method, which analyzes 17O NMR data of water produced by the reactor at different dilution ratios. Moreover, it was found that the LENR process produces stable, 200 nm nanobubbles (density 9.33 × 106 particles/mL for the sample #185r12), which possibly consists of non-condensable gases (O2 and CO2) containing 17O. These persistent nanobubbles could enhance the long-term storage stability of isotopic water. A preliminary in vitro cell assay (MTT) using 17O-enriched water (0.35 mol%) revealed alterations in cancer cell metabolic activity, though further studies are required to evaluate potential biological mechanisms. Overall, this cost-effective production technology may provide 17O-enriched water for physical and biological research.