SOP: ANTIBODY VALIDATION/STAINING Transcription Factor/Nuclear Staining v1

Antibody staining and validation of intracellular and nuclear transcription factors for analysis CyTOF. The protocol outlines the preparation of fresh or cryopreserved cells, viability staining with Cell-ID Cisplatin, Fc receptor blocking, surface antibody staining, fixation and permeabilization, intracellular and nuclear antibody staining, and Iridium intercalation for sample preparation.

Authors

Institutions

Publication Details

Published
2026-10-29
DOI
https://doi.org/10.17504/protocols.io.1wgb1dkmqvpk/v1
Primary Topic
Single-cell and spatial transcriptomics
Type
preprint
Controls
|||
ALL TIME
JAN
FEB
MAR
APR
MAY
JUN
JUL
AUG
SEP
OCT
preprint

SOP: ANTIBODY VALIDATION/STAINING Transcription Factor/Nuclear Staining v1

Eduardo Maurício
2 links
Single-cell and spatial transcriptomics
preprint

SOP: ANTIBODY VALIDATION/STAINING Transcription Factor/Nuclear Staining v1

Eduardo Maurício
preprint en

Abstract

Antibody staining and validation of intracellular and nuclear transcription factors for analysis CyTOF. The protocol outlines the preparation of fresh or cryopreserved cells, viability staining with Cell-ID Cisplatin, Fc receptor blocking, surface antibody staining, fixation and permeabilization, intracellular and nuclear antibody staining, and Iridium intercalation for sample preparation.

UCLA Health (US)
Single-cell and spatial transcriptomics
DOIOpen Access PDFOpenAlex
AI Navigator

Ask Laika to Summarize, Analyze, and Connect papers live on the map.

Summarize Papers & Methodologies

Extract key findings, datasets, and comparative methods across publications.

Benchmark Rankings & Visual Analytics

Rank top research institutions, authors, funders, topics, and journals by Field-Weighted Citation Impact (FWCI) and paper volume with instant charts.

Connect Distant Disciplines

Bridge topological clusters on the map to find hidden collaborative intersections.

Laika access coming soon
SOP: ANTIBODY VALIDATION/STAINING Transcription Factor/Nuclear Staining v1 — Eduardo Maurício · (2026) | TGRS Research Map | TGRS