Airborne and Surface Bacteria in ORs : Integration of ASHRAE ‐Compliant Ventilation and Active UV ‐C Air Treatment

Bacteria in ORs increase surgical site infection risk. The American Society of Heating, Refrigerating and Air-Conditioning Engineers Standard 170 defines OR ventilation standards, but the added benefit of in-room supplemental ultraviolet C air treatment remains uncertain. We compared bacterial counts before and after installing active ultraviolet C air treatment in an OR with Standard 170-compliant ventilation, using a similar OR as a control with Standard 170-compliant ventilation alone. After installation, the intervention OR showed 80% fewer airborne bacteria and 61% fewer settled surface bacteria. The control OR showed a 219% increase in airborne colony-forming units per cubic meter and a 24% increase in settled surface bacteria over the same period, reflecting potential confounders. Although the intervention was associated with lower bacterial levels in two assays, more evidence is needed given the limited number of ORs tested and the variation in the control OR.

Authors

Publication Details

Journal
AORN Journal
Published
2026-10-28
DOI
https://doi.org/10.1002/aorn.70176
Primary Topic
Infection Control and Ventilation
Type
article
Field-Weighted Citation Impact
0.00
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article

Airborne and Surface Bacteria in ORs : Integration of ASHRAE ‐Compliant Ventilation and Active UV ‐C Air Treatment

Isabelle Clark, Kellee Necaise, Linda D. Lee, Lauren Nemeth et al.
AORN Journal
Infection Control and Ventilation
article

Airborne and Surface Bacteria in ORs : Integration of ASHRAE ‐Compliant Ventilation and Active UV ‐C Air Treatment

Isabelle Clark, Kellee Necaise, Linda D. Lee, Lauren Nemeth, Russell N. Olmsted, Conor Donnelly, Kristen Metzger, L. Michelle Cleveland‐Hall, Jennifer McNary Smith, Matthew Toldy, Sarah Bomgaars, Haley Hatfield
article en

Abstract

Bacteria in ORs increase surgical site infection risk. The American Society of Heating, Refrigerating and Air-Conditioning Engineers Standard 170 defines OR ventilation standards, but the added benefit of in-room supplemental ultraviolet C air treatment remains uncertain. We compared bacterial counts before and after installing active ultraviolet C air treatment in an OR with Standard 170-compliant ventilation, using a similar OR as a control with Standard 170-compliant ventilation alone. After installation, the intervention OR showed 80% fewer airborne bacteria and 61% fewer settled surface bacteria. The control OR showed a 219% increase in airborne colony-forming units per cubic meter and a 24% increase in settled surface bacteria over the same period, reflecting potential confounders. Although the intervention was associated with lower bacterial levels in two assays, more evidence is needed given the limited number of ORs tested and the variation in the control OR.

AORN JournalVol. 124(5)
Good health and well-being
Openalex Percentile: Top 12%
Infection Control and Ventilation
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