Ultra-long DNA Extraction for UMI-Tagged rRNA Operon Amplification Nanopore sequencing v2
Recovery of high-molecular-weight (HMW) DNA at sufficient yield is a key prerequisite for long-read amplicon sequencing of the full 16S–ITS–23S rRNA operon, yet most commercial extraction kits are optimized either for yield or for fragment length, rarely both. Here we describe two sequential extraction workflows—DoubleBeads (DB) and EnzyBeads (EB)—that adapt established commercial protocols to simultaneously maximize HMW DNA recovery and total DNA yield from environmental and microbial community samples.
Authors
- Ameet J. Pinto (ORCID: https://orcid.org/0000-0003-1089-5664)
- Kaiqin Bian (ORCID: https://orcid.org/0000-0002-5682-3167)
- Jinhao Yang
- Zijun Meng
Institutions
- Georgia Institute of Technology (US)
Publication Details
- Published
- 2026-10-27
- DOI
- https://doi.org/10.17504/protocols.io.n2bvjkk8wgk5/v2
- Primary Topic
- Genomics and Phylogenetic Studies
- Type
- preprint
Funders
- National Science Foundation
- Georgia Institute of Technology