Expansion microscopy of paraffin-embedded mouse testis allows enhanced detection of spatially restricted somatic and germ cell proteins

Testis expansion microscopy (testis ExM), informed by newly developed best-practices and a semi-automated analysis pipeline, demonstrates testis ExM as a powerful platform to define subcellular protein localization using standard light microscopy.

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Publication Details

Journal
Biology of Reproduction
Published
2026-10-26
DOI
https://doi.org/10.1093/biolre/ioag240
Primary Topic
Advanced Fluorescence Microscopy Techniques
Type
article
Field-Weighted Citation Impact
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article

Expansion microscopy of paraffin-embedded mouse testis allows enhanced detection of spatially restricted somatic and germ cell proteins

Ashley Di Agostino, Elizabeth M. Snyder, Sarah Potgeiter
Biology of Reproduction
Advanced Fluorescence Microscopy Techniques
article

Expansion microscopy of paraffin-embedded mouse testis allows enhanced detection of spatially restricted somatic and germ cell proteins

Ashley Di Agostino, Elizabeth M. Snyder, Sarah Potgeiter
article en

Abstract

Testis expansion microscopy (testis ExM), informed by newly developed best-practices and a semi-automated analysis pipeline, demonstrates testis ExM as a powerful platform to define subcellular protein localization using standard light microscopy.

Biology of Reproduction
Rutgers, The State University of New Jersey (US)
Openalex Percentile: Top 19%
Advanced Fluorescence Microscopy Techniques
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